Part:BBa_K4716994:Design

NSP4 (the Novel Signal Peptide 4)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 48
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We decide to use this part because we need to make the 33-mer enzyme released from the gut flora, the NSP4 peptide is the signal peptide let the enzyme secret out together with it.

Source

We take the NSP4 sequence from 2019 UM_Macau iGEM team, part name: BBa_K3033012